常用载体及通用测序引物

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载体大小(bp) 2984 11100

Vector Primer(F) Primer(R) 抗性

EZ-T LZRS-RfA p3xFLAG CMV14

M13-47 LZRS-F

RV-M LZRS-R

Amp+

CMV30(834bp) CMV24(1151bp) 6310

p3XFlag-mycCMV-F(852bp)/PCEP-F(8

R(hGH polyA)(1217bp)

-CMV-24 96bp) pAAV-MCS

F(β-globin)(1192bp)

R(hGH polyA)(1521bp)

4752

4650

pAc5.1/V5-H

Ac5-Profor(2525bp)

is A

pAc5.1/V5-H

Ac5-Profor(2525bp)

is B

pAc5.1/V5-H

Ac5-Profor(2525bp)

is C pAcGP67_A pAcGP67_B pAcGP67_C pACT

PH-Profor(4016bp) PH-Profor(4016bp) PH-Profor(4016bp) T7(1061bp)

5'AD(同GAL4AD)(5134bp)

BGH(2768bp)/EBVrev(2844bp) Amp+ 5375

BGH(2772bp)/EBVrev(2848bp) Amp+ 5379

BGH(2764bp)/EBVrev(2840bp) Amp+ 5371

pAcGP67rv(4329bp) pAcGP67rv(4329bp) pAcGP67rv(4329bp) T3(1420bp)

Amp+ Amp+ Amp+ Amp+

9761 9761 9761 5566

pACT2

3'AD(4939bp)/pGAL4-Adrv(496Amp+ 4bp)(pGAL4-Adrv离MCS少于

8117

20bp，优先用3'AD)

PACYCDuet-DuetUP1(3949bp)

1(MCSⅠ)

PACYCDuet-DuetUP2(189bp)

1(MCSⅡ)

DuetDOWN1(209bp) Chl+ 4008

T7ter(468bp) Chl+ 4008

pAdTrack

pEGFP-N-3’(同EGFP-Nr

CMV-F(1433bp)

ev)(1294bp)

pEGFP-N-3’(同EGFP-Nr

EBVrev(1982bp)

ev)(1272bp) 5'AOX

3'AOX

Amp+/Ka

8111

n+

pAdTrack-CMV PAO815

Kan+ 9010

7700

pAS2-1

5'BD(同GAL4BD)(5894b3'BD(6112bp)/pGAL4-BDrv(604

Amp+

6bp)(优先用3'BD) p)

pB42ADF(787bp)

pB42ADR(924bp)

Amp+

8392

pB42AD 6449

pLXSN-F(同pLXSNfw)

PBABE-IRES

(1027bp)/PBABE5'(1063b无反向引物

-Egfp

p)/pBMN5'(1416bp) pBabe-puro

pBabe5'(1318bp)

pBabe3'(1429bp)

5944

5169

pBACPAK8

BAC1(1181bp)/PH-Profor

BAC2(1374bp)/BaculoVrev(137

(1174bp)/BaculoVfor(121

5bp)

1bp)

Amp+ 5538

pBADfw(211bp)/Xpressfo

pBAD/His A r(375bp)(优先用pBADfpBADrv(514bp)

w)

M13F(3161bp)/T7(3188bp)/M13-47(3135bp) 35S(5735bp)

4102

pBBR1MCS

M13R(3390bp)/T3(3352bp)/RV-Amp+

M(3409bp) 无

4707

pBI121 14758

pBK-CMV T7(975bp)/M13F(951bp) T3(1159bp)/M13R(1196bp) Amp+ 4518

pBluescript I

M13F(599bp)/T7(626bp)

I KS (-)

pBluescript I

M13F(599bp)/T7(626bp)

I KS (+)

pBluescript I

M13F(599bp)/T7(626bp)

I SK (-)

pBlueScript I

M13F(599bp)/T7(626bp)

I SK(+) pBluescript KS (-) pBluescript KS (+)

T3(792bp)/M13R(829bp) 2961

T3(792bp)/M13R(829bp) 2961

T3(792bp)/M13R(829bp) 2961

T3(792bp)/M13R(829bp) 2961

M13F(602bp)/T7(626bp) T3(790bp)/M13R(828bp) 2958

M13F(602bp)/T7(626bp) T3(790bp)/M13R(828bp) 2958

pBluescript S

M13F(602bp)/T7(626bp)

K (-)

pBluescript S

M13F(602bp)/T7(626bp)

K (+) pBSN-T pBudCE4.1(MCSI) pBudCE4.2(MCSⅡ) pBV220

T7

CMV-Profor(552)/T7(638)

T3(790bp)/M13R(828bp) 2958

T3(790bp)/M13R(828bp) 2958

T3

c-mycrev(742bp)/EBVrev(842bp)

Amp+

4595

PEF-F(2999) BGH(3235) 4595

PBV220F(3592bp) PBV220R(93bp) 3667

pCAMBIA13

P1 (11472bp)

00

P2 (2728bp) 11566

pCAMBIA13

P1 (10935bp)

01

pCAMBIA13

P1(11454bp)

04

pCAMBIA23M13F(8418bp)/M13R(-400 8) (8313bp)

P2 (11149bp) 11849

P2(11668bp) 12361

M13F(-47)(8461bp)/ M13R(834

9bp)

8742

pCANTAB_5pCANTAB5-S1(2265bp)/pCANTAB5-S6(2471bp)/pCANE pCANTAB5-R1(2222bp) TAB5-R2(2504bp) pCAT3-enhan

RVP3(4215bp)

cer pCDFDuet

T7(214bp)

4517

无 Amp+ 4273

T7ter(467bp) 3781

pCDH-CMV-CMV-F(2164bp)/PCEP-FMCS-EF1-Pu(2208bp)/pLNCX-F(2210ro bp)

无 7384

pcDNA1.1

CMV-F(2076bp)/T7(2170

无

bp) M13F/T7

M13R/SP6

4000

pcDNA2.1 3013

pcDNA3.0

pcDNA3fw(841bp)/CMV-SP6(1016bp)/BGH(1053bp)

Profor(777bp)/T7(863bp)

T7(863bp)/CMV-Profor(7

BGH(1039bp)

77bp)/pcDNA3fw(841bp)

T7(863bp)/CMV-Profor(7

BGH(1039bp)

77bp)/pcDNA3fw(841bp)

5446

pcDNA3.1 (-) pcDNA3.1 (+)

5428

5428

pcDNA3.1/HiT7(863bp)/CMV-Profor(7

BGH(1122bp)

s A 77bp) pcDNA3.1/HiT7(863bp)/CMV-Profor(7BGH(1122bp)

Amp+ 5514

Amp+ 5514

s B 77bp)

pcDNA3.1/HiT7(863bp)/CMV-Profor(7

BGH(1122bp)

s C 77bp) pcDNA3.1/myc-His A pcDNA3.1/myc-His B pcDNA3.1/myc-His C pcDNA3.1/V5-His A pcDNA3.1/V5-His B pcDNA3.1/V5-His C

T7(863bp)/CMV-Profor(7BGH(1099bp)/c-mycrev(1020b77bp) p) T7(863bp)/CMV-Profor(7BGH(1099bp)/c-mycrev(1020b77bp) p) T7(863bp)/CMV-Profor(7BGH(1099bp)/c-mycrev(1020b77bp) p) T7(863bp)/CMV-Profor(7

BGH(1108bp)/V5rev(1035bp)

77bp)

T7(863bp)/CMV-Profor(7

BGH(1108bp)/V5rev(1035bp)

77bp)

T7(863bp)/CMV-Profor(7

BGH(1108bp)/V5rev(1035bp)

77bp)

Amp+ 5514

Amp+ 5494

Amp+ 5494

Amp+ 5494

Amp+ 5502

Amp+ 5502

Amp+ 5502

pcDNA3.1/ZeT7(863bp)/CMV-F(769bo(-) p) pcDNA3.1/ZeT7(863bp)/CMV-F(769bo(+) p) pcDNA3.3/TOPOTA pcDNA4/HisMax A pcDNA4/HisMax B

BGH(1038bp) Amp+ 5014

BGH(1039bp) Amp+ 5015

CMV-F(584bp)/PCEP-F(6

无

28bp)/pLNCX-F(630bp)

Amp+ 5407

Xpressfor(1119bp) BGH(1282bp) Amp+ 5258

Xpressfor(1119bp) BGH(1282bp) Amp+ 5258

pcDNA4/His

Xpressfor(1119bp) BGH(1282bp) Amp+ 5258

Max C pcDNA4/HisMax-TOPO pcDNA4/HisMax-TOPO/lacZ

Xpressfor(1119bp) BGH(1302bp) Amp+ 5274

Xpressfor(1119bp) BGH(4482bp) Amp+ 8354

pcDNA4/mycCMV-Profor(777bp)/T7(8BGH(1103bp)/ c-mycrev(1024b-His A 63bp) p) pcDNA4/mycCMV-Profor(777bp)/T7(8BGH(1103bp)/ c-mycrev(1024b-His B 63bp) p) pcDNA4/mycCMV-Profor(777bp)/T7(8BGH(1103bp)/ c-mycrev(1024b-His C 63bp) p)

TREfor(908bp)/CMV-Pro

BGH(1106bp)

for(777bp)

Amp+ 5079

Amp+ 5079

Amp+ 5079

pcDNA4/TO Amp+ 5078

pcDNA4/V5-CMV-Profor(777bp)/T7(8

BGH(1117bp)/V5rev(1044bp)

His A 63bp) pcDNA4/V5-CMV-Profor(777bp)/T7(8

BGH(1117bp)/V5rev(1044bp)

His B 63bp) pcDNA4/V5-CMV-Profor(777bp)/T7(8

BGH(1117bp)/V5rev(1044bp)

His C 63bp)

CMV-Profor(777bp)/T7(8

BGH(1039bp)

63bp)

Amp+ 5092

Amp+ 5092

Amp+ 5092

pcDNA5/FRT Amp+ 5069

pcDNA6/mycCMV-Profor(777bp)/T7(8BGH(1099bp)/c-mycrev(1020b-His A 63bp) p) pcDNA6/mycCMV-Profor(777bp)/T7(8BGH(1099bp)/c-mycrev(1020b-His B 63bp) p)

Amp+ 5126

Amp+ 5126

pcDNA6/mycCMV-Profor(777bp)/T7(8BGH(1099bp)/c-mycrev(1020b-His C 63bp) p) pcDNA6/V5-CMV-Profor(777bp)/T7(8

BGH(1121bp)/V5rev(1048bp)

His A 63bp) pcDNA6/V5-CMV-Profor(777bp)/T7(8

BGH(1121bp)/V5rev(1048bp)

His B 63bp) pcDNA6/V5-CMV-Profor(777bp)/T7(8

BGH(1121bp)/V5rev(1048bp)

His C 63bp) pcDNAII

T7(2997bp)

SP6(152bp)

Amp+ 5126

Amp+ 5148

Amp+ 5148

Amp+ 5148

3013

pcDNA™6.2-GW/EmGFP-miRNA-F miR pCEP4

EBVrev(367bp)

miRNA-R Amp+ 5699

pCEP-F(500bp) Amp+ 10410

pCI(neo)

17Base(996bp)/T7(1067b

p)(17Base在T7之前) pCEBVrev(1206bp)/T3(1158bp) I-neoF(886bp)

T3(1158bp)

Amp+ 5472

pCMS-EGFP T7(1067bp) Amp+ 5541

pCMV-3Tag-T3(621bp)/PFLAG-CMV-T7(906bp)/BGH(952bp)

4A F(536bp)

CMV-F(853bp)/PCEP-F(8

无

97bp)

pCMV5R

4233

pCMV5 Amp+ 4657

pCMV5-Flag pCMV5F Amp+

PCMV6

V1.5(815bp)/CMV-Profor

XL39(1077bp)/HGHrev(1022bp) Amp+

(861bp)

CMV-F(882bp)/T7(952bp)/SP6(952bp)

M13R(1048bp)/HGHrev(1089bp)

4665

PCMV6-XL4 Amp+ 4707

PCMV-AC V1.5 XL39(同CMV-24) 5939

pCMV-HA

CMV-Profor(485bp)/TRE

EBVrev(965bp)

for(570bp)

R(hGH polyA)

Amp+ 3782

pCMV-MCS F(β-globin) 4492

pCMV-F(794bp)/CMV-Pr

pCMV-R(965bp)/EBVrev(973b

pCMV-MYC ofor(490bp)/TREfor(570b

p)

p) pCMV-Sport1

pCMV-Sport6

Amp+ 3791

T7(646bp)

SP6(806bp)/pLNCX-F(924)/CM

Amp+

V-F(970bp)(优先用SP6)

4380

M13F(663bp)/T7(691bp) SP6(872bp)/M13R(899bp) 4397

pCMV-Tag(K

T3(621bp)

AN+) pCMV-Tag1(KAN+) pCMV-Tag2(KAN+)

T7(840bp) Amp+ 4219

T3(621bp) T7(840bp) 4219

T3(621bp) T7(840bp) 4219

pCMV-Tag-2

T3(621bp)

B

pCMV-Tag3(KAN+)

T7(845bp) 4323

T3(621bp) T7(847bp) 4219

pCMV-Tag-3

T3(621bp)

B

pCMV-Tag4(KAN+)

T7(846bp) 4300

T3(621bp) T7(840bp) 4219

pCMV-Tag5

T3(621bp) T7(843bp) 4219

(KAN+)

PCMX-GAL4

CMV-F

(MCS区)

CMV-R 4500

pCOLADuet-T7(214bp)/DuetUP2(189b

T7ter(467bp)

p)(优先用T7) 1(MCS2) pcoldⅡ pcoldⅢ pcoldⅣ pcoldI pcoldI pcoldIII

pcoldI-F(208bp) pcoldI-F(208bp) pcoldI-F(208bp) pcoldI-F(208bp) pcoldI-F(208bp) pcoldI-F

pcoldI-R(387bp) pcoldI-R(374bp) pcoldI-R(356bp) pcoldI-R(404bp) pcoldI-R(404bp) pcoldI-R pCold-SUMO-R pCold-SUMO-R

T7(425bp)/M13F(449bp)/M13-4

7(475bp)

M13F(406bp，)/T7(382bp)

Kan+ 3719

Amp+ Amp+

4392 4377 4377 4407 4407 4751 4751 5769

pCold-SUMO pCold-SUMO-F pCold-TF

pCold-TF-F1

pCR blunt IISP6(239bp)/M13R(205b TOPO p)/RV-M(186bp) pCR2.1 pCR2.1-TOPO

M13R(205bp)

Amp+/Ka

3512

n+

3907

M13R(205bp)

M13F(409bp)/T7(385bp)(T7离Amp+/Ka

3909

MCS只有6bp，优先用M13F) n+

Amp+/Ka

5060

n+

5020

pCR3.1 T7(638bp) BGH(831bp)

pCR3.1-Uni T7(638bp) BGH(790bp)

pCR4-TOPO

T3(243bp)/M13R(205bp)/T7(374bp)/M13F(371bp)/M13-4RV-M(186bp) 7(397bp) Amp+/Ka

3956

n+

PCRE-LUC pCR-ScriptSK

RVP3(4976bp) GLP2(327bp) 5034

T7/M13F T3/M13R

Amp+/Ka

n+

pCS2+ (MCS

SP6(35bp)

I)

pCS2+ (MCS

pSG5rv(222bp)

II) pDBLEU

pDBLeu-F(6137bp)

EBVrev(188bp) Amp+ 4095

T3(409bp) Amp+ 4095

pDBLeu-R(6395bp) 9903

pDEST15 GaT7(25bp)/PGEX-F(721bp)

T7ter(2579bp)

teway /pGEXrv(5270bp)

PH-Profor(43bp)/PGEX-F

无

(777bp)/EBVrev(2771bp)

Amp+ 7013

PDEST20 Amp+ 7066

PDEST22

PDEST22-F(4610bp)/pDEPDEST22-R(6539bp)/pDEST22-Amp+

ST22-F1998(4540bp) R4000(6627bp) M13F(4065bp)/T7(4089bp)

8923

pDNR-LIB(MCSA) pDNR-LIB(MCSB) pDONR/Zeo pDONR201 pDONR207 pDONR221

M13R(385bp) 4200

EBVrev(253bp) M13R(385bp) 4200

M13F(536bp) SeqL-A(286bp) SeqL-A(5567bp) M13F(536bp)

M13R(3044bp) SeqL-B(2789bp) SeqL-B(2485bp) M13R(3044bp)

4291 4470 5585 4762

pDrive T7(239bp)/M13R(204bp) SP6(418bp)/M13F(449bp)

Amp+/Ka

3851

n+

pDsRED1-C1

pDsRED-ex-C1-F(1222bp)

无 4686

pDSRED1-N1

CMV-Profor(519bp) RFP-Nrev(751bp) 4692

pDsRED2-C1

pDsRED-ex-C1-F(1219bp)

无反向引物 4675

pDSRED-N1 pEGFP-N-5' pEarlygate102

PEarlygate201

pEasy-blunt

pDSRED-N1-R Kan+

35S(2580bp) 12453

35S(2580bp) 11778

M13R(205bp) M13F(406bp)/T7(382bp) T7ter(440bp)(离MCS不到20bp)

T7ter(5327bp)

3929

pEasy-E1 T7(209bp) 5715

pEasy-E2 T7(5117bp) 5393

pEasy-T1

M13R(205bp)/RV-M(186M13F(405bp)/M13-47(431bp)

(优先用RV-M和M13-47) bp)

M13R(205bp)/RV-M(186M13F(306bp)/M13-47(332bp)(优先用RV-M和M13-47) bp)

M13F(2996bp)/T7(3036b

p删几个碱基后才能找到，最好不用) EGFP-Cfor(1219bp)

3928

pEasy-T1 Simple

3829

pEasy-T3

M13R(214bp)/SP6(180bp)(优先用M13引物)

3036

pECFP-C1 SV40-pArev(1576bp) 4731

pECFP-N1

CMV-Profor(524bp)/pEGpEGFP-N-3’(同EGFP-Nrev)(74

FP-N-5’(545bp) 5bp)

4733

pEF/myc/cytoPEF-F(同EF-1aForward)/ER/mito/nuc (1133bp)

PEF-F(同EF-1aForward)(1133bp)

pCDNA3.1R(1483bp)/c-mycrev(1405bp)

5603

pEF/myc/ER BGH(1483bp) Amp+ 5603

pEGFP(3'MC

pEGFP-C-5’(942bp)

S)

无反向引物 3355

pEGFP(5'MCM13R(距第一个MCS位pEGFP-N-3’(同EGFP-Nrev)(35

点29bp)(205bp) S) 5bp)

EGFP-Cfor(1219bp)/pEGSV40-pArev(1575bp)/pEGFP-C-FP-C-5(1267bp，离MCS3(1410bp，离MCS较近，不建较近，不建议使用) 议使用) EGFP-Cfor(1219bp)/pEGFP-C-5(1267bp离MCS较近，不建议使用)

SV40-pArev(1579bp)/pEGFP-C-3(1434bp，离MCS较近，不建议使用)

3355

pEGFP-C1 4731

pEGFP-C2 4735

pEGFP-C3

EGFP-Cfor(1239bp)/pEGSV40-pArev(1571bp)/pEGFP-C-FP-C-5(1265bp，离MCS3(1425bp，离MCS较近，不建较近，不建议使用) 议使用) EGFP-Cfor(1219bp)

SV40-pArev(1650bp)

4727

pEGFP-F 4806

pEGFP-N1

CMV-Profor(519bp)/pEGpEGFP-N-3’(同EGFP-Nrev)(74

FP-N-5'(566bp) 5bp)

pEGFP-N-3’(同EGFP-Nrev)(74

9bp)

pEGFP-N-3’(同EGFP-Nrev)(74

1bp)

4733

pEGFP-N2 CMV-Profor(524bp) 4737

pEGFP-N3 CMV-Profor(524bp) 4729

pENTR/D (-TOPO) pET-*(His)

M13F(536bp)/SeqL-A(481bp) T7(4010bp)

M13R(861bp)/SeqL-B(894bp) Kan+ 2580

T7Ter(4191p) Kan+ 4636

pET21a T7(5117bp) T7Ter(5357bp) 5443

pET22a(AMP

T7

+)

pET22b(AMP

T7(5117bp)

+)

pET22c(AMP

T7

+) pET-24(+) pET-24a(+) pET-24b(+) pET-24c(+) pET-24d(+) pET28a(+) pET28b(+) pET28c(+)

T7(4984bp) T7 T7 T7 T7 T7 T7 T7

T7Ter 5493

T7Ter(5426bp) 5493

T7Ter 5493

T7Ter(5169bp) T7Ter T7Ter T7Ter T7Ter T7Ter T7Ter T7Ter

5236 5236 5236 5236 5369 5369 5369

pET-30_Ek/L

T7

IC

pET-30_Xa/L

T7

IC pET-30a(+) pET-30b(+) pET-30c(+)

T7(4988bp) T7 T7

T7Ter Amp+ 5439

T7Ter Amp+ 5448

T7ter(5355bp) T7Ter T7Ter

5422 5422

pET-32a pET-32b pET-32c pET-33b(+) pET-34b(+) pET-3a pET-3b pET-3c pET-3d pET-40b(+)

S.tag(5631bp) S.tag S.tag T7(4984bp) T7 T7(1bp) T7(1bp) T7(1bp) T7(1bp) S.tag(5888bp)

S.tag(5603bp)/PGEX-F(5455bp)

S.tag(5603bp)/PGEX-F(5455bp)

S.tag(5603bp)/PGEX-F(5455bp)

S.tag(5603bp)/PGEX-F(5455bp)

S.tag(5603bp)/PGEX-F(5455bp)

S.tag(5603bp)/PGEX-F(5455bp)

T7ter(5833bp) T7ter T7ter T7ter(5316bp) T7ter T7ter(186bp) T7ter(185bp) T7ter(184bp) T7ter(183bp) T7ter(6123bp)

Kan+ Kan+ Amp+ Amp+ Amp+ Amp+ Kan+

5900 5900 5900 5383 5918 4640 4639 4638 4637 6190

pET-41a(+) T7ter(5866bp) Kan+ 5933

pET-41b(+) T7ter(5865bp) Kan+ 5932

pET-41c(+) T7ter(5867bp) Kan+ 5934

pET-42a(+) T7ter(5863bp) Kan+ 5930

pET-42b(+) T7ter(5862bp) Kan+ 5929

pET-42c(+) T7ter(5864bp) Kan+ 5931

pET-43.1a(+) 无 pET-43.1b(+) 无 pET-43.1c(+) 无

S.tag/T7(如果S.tag被切

pET-44_Ek/L

除，可以用T7，正常情

IC

况下不建议使用T7)

T7ter(7209bp) T7ter(7207bp) T7ter(7163bp)

7275 7274 7230

T7ter Amp+ 7331

pET-44a(+)

S.tag(6545bp)/T7(4870bp,如果S.tag被切除，可以

T7ter(7244bp)

用T7，正常情况下不建议使用T7)

S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7) S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7) T7(4870bp)

Amp+ 7310

pET-44b(+) T7ter Amp+ 7310

pET-44c(+) T7ter Amp+ 7310

pET-45b(+) pET46-Ek/LIC pET49b pET-49b(+)

T7ter(5193bp) Amp+ 5260

T7(4870bp) T7ter(5133bp) Amp+ 5200

PGEX-F PGEX-F(5455bp)

T7ter T7ter(5859bp)

5926 5926

pET50b(+)

S.tag(6545bp)/T7(4751bp，如果S.tag被切除，可

T7ter(6666bp)

以用T7，正常情况下不建议使用T7) T7(3711bp)

T7ter(3896bp)

6733

pET-9a Kan+ 4341

pET-9b pET-9c

T7(3711bp) T7(3711bp)

T7ter(3895bp) T7ter(3894bp)

Kan+ Kan+

4340 4339

pETDuet-1(M

pBRrevBam(5297bp)

CSI)

DuetDOWN1(208bp) Amp+ 5420

pETDuet-1(MDuetUP2(189bp)/T7(214b

T7ter(467bp)

CSII) p)

pEGFP-C-3'(1429bp)/SV40-pAr

ev(1575bp)

Amp+ 5420

pEYFP-C1 pEGFP-C-5’(1266bp) Kan+ 4731

pEYFP-N1

pEGFP-N-5’(545bp)/CMV-Profor(519bp) BaculoVfor(4531bp)/PH-Profor(4494bp)

pEGFP-N-3’(同SV40-pArev)(74

Kan+

5bp)

FastBac-rev(4753bp)/SV40-pAre

v(4884bp)

4733

pFastBac Dual (MCSI) pFastBac Dual (MCSII)

5238

HSVtk-pArev(4236bp) p10-Profor(4456bp) 5238

pFastBac-fw(4040bp)/PH-pFastBac HT

Profor(3920bp)/BaculoVfpFastBac-rev(4271bp)

_A

or(3957bp) pFastBac-fw(4040bp)/PH-pFastBac HT

Profor(3920bp)/BaculoVfpFastBac-rev(4271bp)

_B

or(3957bp) pFastBac-fw(4040bp)/PH-pFastBac HT

Profor(3920bp)/BaculoVfpFastBac-rev(4271bp)

_C

or(3957bp)

PH-Profor(3920bp)/Bacul

pFastBac-rev(4191bp)

oVfor(3957bp)

4856

4856

4856

pFastBac1 4776

pFastBac1-Gus

无正向引物 SV40-pArev(6208bp) 6661

pFastBacHT-CAT

无正向引物 SV40-pArev(5046bp) 5500

pFLAG-ATS N26for(18bp) pTrcHis-rv(同pBADrv)(285bp) 5408

pFLAG-CMVpFLAG-CMV-F(863bp)/CpFLAG-CMV-R(1125bp)/CMV-Amp+

1 MV-30(834bp) 24(1144bp) pFLAG-CMVpFLAG-CMV-F(863bp)/CpFLAG-CMV-R(1083bp)/CMV-Amp+ 2 MV-30(834bp) 24(1102bp) pFLAG-MAC N26for(18bp)

pTrcHis-rv(同pBADrv)(225bp) 3'AD(937bp)/pGAL4-Adrv(900b

Amp+

p)

T7(933bp)/3'AD(1045bp)

Amp+

4732

4679

5063

pGAD424 GAL4AD(5'AD)(757bp) 6659

pGADGH GAL4AD(5'AD)(748bp) 5'AD(1827bp)/T7(1905bp)

5'AD(1858bp)/T7(1905bp)

a-FACTOR(同Alphafor)(696bp)/GAP-DH-Profor(455bp)

a-FACTOR(同Alphafor)(696bp)/GAP-DH-Profor(455bp)

a-FACTOR(同Alphafor)(696bp)/GAP-DH-Profor(455bp)

8009

pGADT7 pGAL4-Adrv(2075bp) 7988

pGADT7-Rec 3'AD(2154bp) Amp+ 8059

pGAPZa_A

3'AOX(976bp)/c-mycrev(850bp)

Kan+

/AOX1rev(994bp)

3147

pGAPZa_B

3'AOX(976bp)/c-mycrev(850bp)

/AOX1rev(994bp)

3147

pGAPZa_C

3'AOX(976bp)/c-mycrev(850bp)

/AOX1rev(994bp)

3147

pGBKT7(KAT7(1213bp)/GAL4-BD-Cf3'BD(1494bp)/pGAL4-BDrv(142

Amp+

N+) or(1155bp) 9bp)

7304

pGEM-T(EasM13F(2975bp)/T7(2999by) p) pGene/V5-Hi

pLNCX-F(190bp)/Pgene-s A (GeneS

F(357bp)

witch)

pGene/V5-Hi

pLNCX-F(190bp)/Pgene-s B (GeneS

F(357bp)

witch)

pGene/V5-Hi

pLNCX-F(190bp)/Pgene-s C (GeneS

F(357bp)

witch) pGEX-2T pGEX-2TK pGEX-3X pGEX-4T-1 pGEX-4T-2 pGEX-4T-3 pGEX-5X-1 pGEX-5X-2 pGEX-5X-3 pGEX-6P-1 pGEX-6P-2 pGEX-6P-3

PGEX-F(874bp) PGEX-F(874bp) PGEX-F(874bp) PGEX-F PGEX-F PGEX-F PGEX-F PGEX-F PGEX-F PGEX-F PGEX-F PGEX-F

SP6(158bp)/M13R(192bp) Amp+ 3015

BGH(659bp)/v5rev(586bp) Amp+ 4650

BGH(663bp)/v5rev(590bp) Amp+ 4654

BGH(655bp)/v5rev(582bp) Amp+ 4646

PGEX-R(1016bp) PGEX-R(1037bp) PGEX-R(1002bp) PGEX-R PGEX-R PGEX-R PGEX-R PGEX-R PGEX-R PGEX-R PGEX-R PGEX-R

4948 4969 4952 4969 4970 4968 4972 4973 4974 4984 4985 4983

pGEX-KG pGL2-Basic

PGEX-F GLP1(5564bp)

PGEX-R GLP2(99bp) GLP2(291bp)

5006 5598 6046

pGL2-Control GLP1(6013bp) pGL2-Enhanc

GLP1(5821bp)

er

pGL2-Promot

GLP1(5756bp)

or

pGL3-Basic (MCS I) pGL3-Basic (MCS II)

GLP2(99bp) 5854

GLP2(291bp) 5789

RVP3(4760bp) GLP2(111bp) 4818

无 RVP4(2080bp) 4818

pGL3-Control

RVP3(5198bp)

(MCS I)

pGL3-Control

pLXSNrv(2407bp)

(MCS II)

pGL3-Enhanc

RVP3(5006bp)

er (MCS I)

pGL3-Enhanc

pLXSNrv(2215bp)

er (MCS II)

pGL3-Promot

RVP3(4952bp)

er (MCS I) pGL3-Promot

无

er (MCS II) PGL4.10 pGL4.70

RVP3(4191bp) RVP3(3471bp)

GLP2(303bp) 5256

RVP4(2518bp) 5256

GLP2(111bp) 5064

RVP4(2326bp) 5064

GLP2(303bp) 5010

RVP4(2272bp) 5010

GLP2(130bp) hrRLuc-Nrev(148bp)

Amp+

4242 3522

pGS21a pHEN2 pinpointTM

T7(1bp) M13R(2206bp) PinpointPrimer(325bp)

T7ter(982bp) 无 SP6(468bp)

Amp+

6169 4616 3331

pIRES (MCS

T7 (1067bp)

I)

pIRES (MCS

无

II) pIRES2-EGFP

pIRESrv(1125bp) Amp+ 6092

T3(1776bp)/EBVrev(1824bp) Kan+ 6092

pIRES2-EGFP.P5(531bp)/pIRES2-EGFP.P3(732bp)/IRES-Amp+

CMV-Profor(524bp) R(781bp)

5308

pIRES-hrGFPCMV-Profor(530bp)/ T3

-2a (521bp)

T7(863bp)/CMV-F(769b

p)

T7(620bp)/pBRrevBam(517bp)

无 Amp+ 4988

pIRESneo2 pIRESrv(1326bp) Amp+ 5295

pIVEX 2.3d T7ter(882bp) Amp+ 3560

pJET1.2/blunt

PJET1.2-F(310bp)/T7(305

PJET1.2-R(429bp)

bp)

pJG4-5Forward(807) pKKrv(4534bp)/pBADrv(4512bp)

pKKrv(4534bp)/pBADrv(4512bp)

EGFP-Cfor(3680bp)

pJG4-5Reverse(906)

Amp+ 2974

pJG4-5 6449

pkk223 pKKfw(31bp) 4586

pkk223-3 pKKfw(31bp) 4586

pLEGFP-C1 pLNCX-R(3960bp) 6892

pLenti6/V5 CMV-F(2274bp)/PCEP-FD-TOPO Gat(2318bp)/pLNCX-F(2320

v5rev(2508bp) 6963

eway (ViraPower) pLex

bp)

无 M13F(2507bp) Amp+ 2886

pLEXA

PLEXA-F(2081bp)/LexAfPLEXA-R(2298bp)/pGAL4-BDr

or (2081bp) v (2225bp) SV40(1839bp)/EBVrev(1652bp)

T7(1878bp)/M13F(1923bp)/M13

-47(1949bp)

10166

pLKO.1-TRC 8901

PLL3.7 pLNCX pLNCX2

U6-promoter-ZL(2476bp) PLNCX-F(804bp) CMV-Profor(2841bp)

PLNCX-R(3544bp) pRevTRErv(3033bp)

7650 6620 6133

PLVX-SHRNU6-promoter(human)(238

无

A1 3bp)

PLXSN-F(1574bp)/pLXS

Nfw (1572bp) malE(2587bp) pMAL-C2x-F(2558bp) malE(2587bp) malE(2662bp) T7ter(68bp) RV-M (484bp) M13-47(352bp) RV-M(2707bp)

Amp+ 7758

pLXSN

PLXSN-R(1759bp)/pLXSNrv(17

01bp)

M13-47(2782bp) pMAL-C2x-R(2803bp) pMAL-C5x-R(2846bp) M13-47(2854bp) T7(376bp) M13-47(375bp) RV-M (507bp) M13-47(170bp)

Amp+ Amp+ Amp+ Amp+ Amp+

5874

pMAL-c2E pMAL-C2x pMAL-C5x pMAL-p2X PMCSG7 pMD18-T pMD19-T PMD20-T

6649 6646 5677 6721 5286 2692 2692 2737

pMSCV-hyg pMSCV-neo

pLXSNfw(1332bp) pLXSNfw(1332bp)

pMSCVrev(1672bp) pMSCVrev(1679bp) pMSCVrev(1659bp)

Amp+ Amp+ Amp+

6971 6467 6295

pMSCV-puro pLXSNfw(1332bp) pMT/V5-HisA

pMT/V6-HisB

pMT/V7-HisC pOTB7 pPCR-Script-Amp pPCR-Script-CAM

MT-Profor(814bp) BGH(1038bp)/v5rev(965bp) 3538

MT-Profor BGH/v5rev

MT-Profor BGH/v5rev

M13F(110bp)/SP6(53bp) M13R(252bp)/T7(311bp) 1815

M13-47(573bp)/M13F(59T3(790bp)/M13R(828bp)/RV-M9bp)/T7(626bp) (847bp) M13-47(573bp)/M13F(59T3(790bp)/M13R(828bp)/RV-M9bp)T7(626bp) (847bp) a-FACTOR(同Alphafor)(1152bp)/5’AOX(855bp,

3'AOX(1348bp)

离MCS较远，优先用a-factor)

2961

3399

pPIC9K(AMP+、ZEO+)

Amp+ 9300

pPICZa

5'AOX(同AOX1for)(8543'AOX(同AOX1rev)(1180bp)/C

Amp+

bp) -mycrev(1035bp) 5'AOX(同AOX1for) 5'AOX(同AOX1for)

3'AOX(同AOX1rev)/C-mycrev Amp+ 3'AOX(同AOX1rev)/C-mycrev Amp+ pBADrv(同pTrcHis-rv)(483bp) pBADrv(同pTrcHis-rv)

3329

pPICZb pPICZc

3329 3329 4751 4751

pPROEXHTa RV-M(234bp) pPROEXHTb RV-M

pPROEXHTc RV-M pBADrv(同pTrcHis-rv) 4751

PPZP111

M13R(8467bp)/RV-M(84

M13F(8571bp)/M13-47(8597bp)

48bp)

M13R(6697bp)/RV-M(66

M13F(6801bp)/M13-47(6827bp)

78bp)

pLNCX-F(2108bp) pQE30-F(65bp)/TypeIII/IV(69bp) pQE30-F(65bp)

IRES-R(2404bp)

Amp+

8909

PPZP201 7132

PQCXIN 7381

pQE30 pQE30-R(243bp) Amp+ 3461

pQE40 pQE30-R(813bp) Amp+ 4031

pQE80L

BLA-Prorev(4573bp)Type

III/IV(69bp)pQE30-F(65bpQE30-R(243bp) p)

4751

PQE-trisystem

TriExup(1021bp) TriExdowm(1529bp) Amp+ 5766

pQE-TriSyste

m His.Strep PCAG-F(1012bp) 1

Baculovirs_rev(1812bp)/BaculoVrev(1931bp)

5760

PRK5-flag SP6(828bp)

pSG5rv(同SV40-pArev)(1030bp)

4661

pRNA-U6.1/Neo

T7 BGH Amp+

pRS424

M13F(1840bp)/T7(1867b

无

p)/pBluescriptKS(1910bp) T7(20bp)/Xpressfor(139bp)

DuetUP1(3771bp)

5616

pRSET T7TER(317bp) 2897

pRSFDuet-1

DuetDOWN1(208bp) 3829

(MCSI) pRSFDuet-1(MCSII)

DuetUP2(189bp) T7TER(467bp) 3829

pSectag(v5/H

T7(863bp)

is/a,b,c) pSHUTTLE-CMV

BGH(1154bp) Kan+ 5185

CMV-F(857bp)

PSHUTTLE-CMV-R(1031bp)/S

V40-pArev(同pSG5rv)(1173bp)

7469

pSilencer_4.1

Psilencer-4.1-F(377bp)

-CMV_neo pSilencer2.0-U6 pSilencer3.1-H1hygro#

Psilencer-4.1-R(639bp) 5590

T7(271bp)

2.0REV/M13F(696bp)(M13F比

Amp+

2.0REV离MCS近30bp左右)

3075

M13-47(352bp) 3.0REV(1163bp) 5597

pSIREN-F(6188bp)/PBA

pSIREN-Retr

BE5'(6141bp)/PLXCN/pLpMSCVrev(252bp)

oQ

XSN-F(6105bp)

SP6(2982bp)/pBRrevBam(2673bp,离MCS稍远，优M13R(83bp)/RV-M(102bp) 先用SP6)

SP6(2989bp)/pBRrevBam(2679bp,离MCS稍远，优M13R(89bp)/RV-M(108bp) 先用SP6) SP6(2446bp)

T7(118bp)

6445

pSP64 Amp+ 2999

pSP65 Amp+ 3005

pSP72 Amp+ 2462

pSport1

SP6(165bp)/M13F(139bp)T7(319bp)/M13R(353bp)/RV-M/M13-47(113bp) (372bp) T7(24bp)/M13R(3839bp) SP6(202bp)/M13F(233bp)

4110

pSTBlue-1 Amp+ 3851

PSTV28 pSUPER pT-Adv

M13-47(1640bp) T7(626bp)/M13F(599bp) M13R(205bp)

pTarget.F(1181bp)(在T7上游)/T7(1229bp) M13F

RV-M(1789bp)

T3(1005bp)/M13R(1043bp) M13F(405bp)/T7(381bp)

2999 3176 3906

pTargeT™ pTarget.R(1454bp) 5670

PTG19-T M13R

M13F(同TrxReverse/M13F-20)(3986bp)

M13F(同TrxReverse/M13F-20)(3984bp)

M13F(同TrxReverse/M13F-20)(3988bp)

2880

pThioHis A TrxForward(3777bp) 4365

pThioHis B TrxForward(3777bp) 4363

pThioHis C TrxForward(3777bp) 4367

ptkLUC (MC

EBVrev(4428bp)

SI 1-270) ptkLUC (MC

无

SII：2100) pTRC99a-c pTrcHis A pTrcHis B pTrcHis C

pTRC99C-F(146bp) Xpressfor(452bp) Xpressfor Xpressfor

LucNrev(322bp) Amp+ 4711

M13R(2200bp) Amp+ 4711

pTRC99C-R(385bp) 无无无

4176 4414

PTRE(clontecPCEP-F(289bp)/pLNCX-h) F(291bp)

无 2605

pTriEx1.1_N

T7(2150bp)/TriExup(2099IRES-R(2608bp)

6664

eo bp)

T7(680bp)/TriplEx-LDrv (678bp) T7/M13R

pTriPLEx2 TriplEx-LD-5´(521bp) 3589

PTZ57R/T M13F 2886

pUC118

RV-M(186bp)/M13R(205

M13-47(334bp)/M13F(308bp)

bp)

RV-M(186bp)/M13R(205

M13-47(334bp)/M13F(308bp)

bp)

M13-47(352bp)/M13F(37

RV-M(500bp)/M13R(481bp)

8bp)

M13-47(352bp)/M13F(37

RV-M(500bp)/M13R(481bp)

8bp) M13F

M13R

3162

pUC119 3162

pUC18 2686

pUC19 2686

pUC20 2705

pUC57-T

M13-47(352bp)/M13F(37

RV-M(524bp)/M13R(505bp)

8bp)

M13F(378bp)/T7 M13F

T7(664bp)/ CMV-Profor(575bp)

M13R(482bp) M13R

Amp+ 2710

pUCm-T pUM-T

Kan+

2773 2726

pVAX1 BGH(841bp) 2999

pwpxl

PEF-F/EF-1aForward(324

无反向引物

5bp)

T7/M13F/M13-47 T7

T3/M13R/RV-M SP6

10501

pWSK29 pXT7 pYES2

5857

pYes2.R(同CYC1-Termi

GAL1-Profor(509bp)/T7(386bp)

nator)(218bp)

GAL1-Profor(352bp)/T7(475bp)

GAL1-Profor(352bp)/T7(475bp)

pYes2.R(同CYC1-Terminator)(743bp)

pYes2.R(同CYC1-Terminator)(743bp)

pYES2/CT 5963

pYES3/CT 5870

TOPO-PENTR-SD

M13F(536bp) M13R(862bp) 2580

1 2 3 4 5 6

1492R 27F 3.0rev 35S 3'AD 3'AOX

引物名称

序列(5'-3')

GGTTACCTTGTTACGACTT AGAGTTTGATCCTGGCTCA GAGTTAGCTCACTCATTAGGC GACGCACAATCCCACTATCC AGATGGTGCACGATGCACAG GGCAAATGGCATTCTGACAT TAAGAGTCACTTTAAAATTTGTATAC

TACCACTACAATGGATGATG GACTGGTTCCAATTGACAAGC CCCTCATAGTTAGCGTAACG TACTATTGCCAGCATTGCTGC

7 3'BD

8 9

5'AD(同GAL4AD) 5'AOX

10 96gIII(同M13-96) 11 a-FACTOR

12 BAC1 13 BAC2 14 BaculoVfor 15 BaculoVrev 16 BGH 17 CMV-24 18 CMV-30 19 CMV-Profor 20 CMV-R 21 C-mycrev 22 DuetDOWN1 23 DuetUP1 24 DuetUP2 25 EBVrev 26 EF-1aForward 27 EGFP-Nrev 28 EGFP-Cfor 29 F(β-globin) 30 GAL4BD(同5'BD)

AACCATCTCGCAAATAAATA ACGCACAGAATCTAGCGCTT TGTTTTCGTAACAGTTTTG AACGCACAGAATCTAGCGC TAGAAGGCACAGTCGAGG TTAGGACAAGGCTGGTGG ATAACCCCGCCCCGTTG ATGGGCGGTAGGCGTG TCGTTGGGCGGTCAGC CTCTTCTGAGATGAGTTTTTG GATTATGCGGCCGTGTACAA GATCTCGACGCTCTCCCT TTGTACACGGCCGCATAATC GTGGTTTGTCCAAACTCATC TCAAGCCTCAGACAGTGGTTC CGTCGCCGTCCAGCTC AGCACCCAGTCCGCCCTGAGC ATTCTGAGTCCAAGCTAGGC TCATCGGAAGAGAGTAG

31 GAP-DH-Profor 32 GLP1 33 GLP2

GTCCCTATTTCAATCAATTGAA TGTATCTTATGGTACTGTAACTG CTTTATGTTTTTGGCGTCTTCCA CGGTAGGTATTGATTGTAATTCTG

CCTCACATTGCCAAAAGACG GCATCGATGAAGAACGCAGC TCCTCCGCTTATTGATATGC GGAAGTAAAAGTCGTAACAAGG CGTCAGCAGAGCTTCACCATTG CGCCAGGGTTTTCCCAGTCACGAC

CAGGAAACAGCTATGACC AGCGGATAACAATTTCACACAGGA

GGTCGTCAGACTGTCGATGAAGCC

TAGTATCCGACGCCGCCATC CATCATAACGGTTCTGGC CACCTGATCCCAGCTTGC CGTCCAGCTCCATGTTGC

34 GPDpromoter-F

35 IRES-R 36 ITS3 37 ITS4 38 ITS5 39 LexAfor

40 M13-47

41 M13R

42 RV-M

43 malE

44 mU6F2(mouse) 45 N26for 46 NOSF-primer 47 NOSR-primer

48 P1 49 P2 50 P12584 51 P17110 52 p10-Profor 53 pAcGP67rv 54 PAS2-1F 55 PAS2-1R

CCAGGCTTTACACTTTATGC GCGATTAAGTTGGGTAACGC TTTTCAGTATCTACGAT TACCACTACAATGGATG CGGACCTTTAATTCAACCC TTGGTTCTTGCCGGGTC TCATCGGAAGCGAGTAG CGTTTTAAAACCTAAGAGTCAC CCAGCCTCTTGCTGAGTGGAGATG

AAGCCGACAACCTTGATTGGAG ACCCTAACTGACACACATTCC CTTTATCCAGCCCTCAC CCATAGCATTTTTATCCATAAG ATCTGTATCAGGCTGAAAATC GGTGATGTCGGCGATATAGG AAGAAGGGCAGCATTCAAAG CTGCGTTCTGATTTAATCTG CCATGATTACGCCAAGCTTTGGAG

56 PB42ADF

57 PB42ADR 58 pBABE3' 59 PBABE5' 60 pBADfw 61 pBADrv 62 pBRrevBam 63 PBV220F 64 PBV220R

65 pCANTAB5-R1

66 pCANTAB5-R2 67 pCANTAB5-S3 68 pCANTAB5-S4 69 pCDNA3.0F 70 pCDNA3.0R 71 pCDNA3.1F 72 PCEP-F 73 pCMV-5R 74 PCMV-F 75 PCMV-R 76 pcoldI-F 77 pcoldI-R 78 pDBLeu-F 79 pDBLeu-R 80 pDsRED-ex-C1-F 81 pDSRED-N1-R 82 pEGFP-C-3' 83 pEGFP-C-5’ 84 pEGFP-N-5'

CGATCTAAAGTTTTGTCGTCTTTC GGTTCAGGCGGAGGTGGCTCTGG CCAGAGCCACCTCCGCCTGAACC CTCTGGCTAACTAGAGAACC GGCAACTAGAAGGCACAGTC CTAGAGAACCCACTGCTTAC AGAGCTCGTTTAGTGAACCG ATTATAGAGGACACCTAGTC TCTAAAAGCTGCGGAATTGT TCCAAACTCATCAATGTATC ACGCCATATCGCCGAAAGG GGCAGGGATCTTAGATTCTG GAATAAGTGCGACATCATCATC GTAAATTTCTGGCAAGGTAGAC TCCCACAACGAGGACTACAC TGAAGCGCATGAACTCCTTG TATGGCTGATTATGATCAGT CATGGTCCTGCTGGAGTTCGTG TGGGAGGTCTATATAAGCAGAG

85 pFASTBAC-F 86 pFASTBAC-R 87 pFastBac-rev 88 HSVtk-pArev 89 pFastBac-fw 90 pFlag-CMV-F 91 pFlag-CMV-R 92 pGAL4-ADrv 93 pGAL4-BDrv 94 pGEXfw 95 pGEXrv 96 PH-Profor 97 PinPointprimer 98 pIRES2-EGFP.P3’ 99 pIRES2-EGFP.P5’ 100 101 102

TGAAGTGGTTCGCATCCTC TGGACAAACCACAACTAGAATG CCTCTACAAATGTGGTATGG GTCTCCTTCCGTGTTTCAG GTCCGAAACCATGTCGTAC GGTAGGCGTGTACGGTGG GCACTGGAGTGGCAACTT GAACTTGCGGGGTTTTTC AATCATAAGAAATTCGCCC GGCAAGCCACGTTTGGTG GAGCTGCATGTGTCAGAGG AAATGATAACCATCTCGC CGTGACGCGGTGCAGGGCG AACGCACACCGGCCTTATTC GTAGGCGTGTACGGTGGGAG

pIRESrv GCCCTAGATGCATGCTCG

pIRES-hrGFPrv GTCCTTATCATCGTCGTCTT

pKKfw AGCGGATAACAATTTCAC

103 104 105 106 107 108 109 110 111 112 113 114 115

pKKrv TCTCTCATCCGCCAAAACAG

pLXSNfw CCCTTGAACCTCCTCGTTCG

pLXSNrv ACTTTCCACACCCTAACTG

pLXSN-F CTTGAACCTCCTCGTTCGAC

pLXSN-R GTTGCTGACTAATTGAGATG

pLNCX-F

AGCTCGTTTAGTGAACCGTCAGATCG

ACCTACAGGTGGGGTCTTTCATTCCC

pLNCX-R

pLEXA-F CGTCAGCAGAGCTTCACCAT

pLEXA-R TAAAACCTAAGAGTCACTTT

pMAL-C2X-F TGCGTACTGCGGTGATCAAC

pMAL-C2X-R CTGCAAGGCGATTAAGTTGG

PPC86-F TATAACGCGTTTGGAATCACT

pQE30-F TGAGCGGATAACAATTTCAC

116 117 118 119 120 121 122 123 124 125 126 127 128

pQE30-R GTTCTGAGGTCATTACTGG

pRevTRErv CCCCCTTTTTCTGGAGAC

pSG5rv GAAATTTGTGATGCTATTGC

pSHUTTLE-CMV-F GGTCTATATAAGCAGAGGTG

pSHUTTLE-CMV-R GTGGTATGGCTGATTATGATCAG

pSilencer4.1-R CGGTAGGCGTGTACGGTG

pTarget.F CCAGGATTTTCCCAGTCAC

pTarget.R GGCTTTACACTTTATGCTTC

pTARGET-seq

TTACGCCAAGTTATTTAGGTGACA

PTRC99C-F TTGCGCCGACATCATAAC

PTRCHISA-F CATGGTATGGCTAGCATGAC

PTRCHISA-R CAGGCTGAAAATCTTCTCTC

pYes2.R/CYC1-Terminator GCGTGAATGTAAGCGTGAC

129 130 131 132 133 134 135 136 137 138 139 140 141

RFP-Nrev GTTCACGGTGCCCTCC

R(hGH polyA) TAGAAGGACACCTAGTCAGA

RVP3 CTAGCAAAATAGGCTGTCCC

RVP4 GACGATAGTCATGCCCCGCG

pCANTAB5-S1

CAACGTGAAAAAATTATTATTCGC

pCANTAB5-S6 GTAAATGAATTTTCTGTATGAGG

SeqL-A GCAGTTCCCTACTCTCGC

SeqL-B CATCAGAGATTTTGAGACAC

SP6 ATTTAGGTGACACTATAG

S.TAG CGAACGCCAGCACATGGACA

SV40 GGAGGCTTTTTTGGAGGC

T3 ATTAACCCTCACTAAAGGGA

T7 TAATACGACTCACTATAGG

142 143 144 145 146 147 148 149 150 151 152 153 154

T7(17base) ACATCCACTTTGCCTTTCTC

T7ter TGCTAGTTATTGCTCAGCGG

tac-Profor AATTAATCATCCGGCTCG

TrxForward TTCCTCGACGCTAACCTG

TrxReverse TGTAAAACGACGGCCAGTGC

TriplEx-LDrv

ATACGACTCACTATAGGGCGAATTGGC

CTCGGGAAGCGCGCCATTGTGTTGG

TriplEx-LD-5'

U6-promoter(human) CCGTAACTTGAAAGTATTTCG

v1.5 GGACTTTCCAAAATGTCG

V5rev ATCGAGACCGAGGAGAGG

WPRE-R CATAGCGTAAAAGGAGCAACA

Xpressfor AGCATGACTGGTGGACAG

pSilencer4.1-F/2.0rev AGGCGATTAAGTTGGGTA

155 156 157 158 159 160 161 162 163 164 165 166

pCDNA3.1R/BGH TAGAAGGCACAGTCGAGG

XL39/CMV-24 ATTAGGACAAGGCTGGTGGG

PEF-F/EF-1aForward TCAAGCCTCAGACAGTGGTTC

pTrcHis-rv/pBADrv ATCTGTATCAGGCTGAAAATC

PTRC99C-R/PBV220R CTGCGTTCTGATTTAATCTG

PPC86-R/pDBLeu-R GTAAATTTCTGGCAAGGTAGAC

M13F/TrxReverse/M13F-20 TGTAAAACGACGGCCAGT

SV40-pArev/pSG5rv GAAATTTGTGATGCTATTGC

CMV-F(与CMV-Profor距离近，可通用)

CGCAAATGGGCGGTAGGCGTG

pEGFP-N-3’(与EGFP-Nrev距离近，可

CGTCGCCGTCCAGCTCGACCAG

通用)

GATGCCTCCTACCCTTATGATGTGCC

GGAGACTTGACCAAACCTCTGGCG

北京市空港工业区B区6号楼

pJG4-5Forward

pJG4-5Reverse

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