. BG11培养基,常用的淡水蓝藻和绿藻培养基(很多微囊藻、衣藻、小球藻、栅藻都可以的,但不能保证是最佳的) Component Amount Stock Solution (1) NaNO3 10mL/L 15.0g/100mLdH2O (2) K2HPO4 1mL/L 4g/100mLdH2O (3) MgSO4·7H2O 1mL/L 7.5g/100mLdH2O (4) CaCl2·2H2O 1mL/L 3.6g/100mLdH2O (5) Citric acid 1mL/L 0.6g/100mLdH2O (6)Ferric ammonium citrate 1mL/L 0.6g/100mldH2O (7) EDTANa2 1mL/L 0.1g/100mldH2O (8) Na2CO3 1mL/L 2.0g/100mldH2O (9) A5 (Trace mental solution )1ml/L H3BO3 2.86g/LdH2O MnCl2·4H2O 1.86g/LdH2O ZnSO4·7H2O 0.22g/LdH2O Na2MoO4.2H2O 0.021g/LdH2O CuSO4. 5H2O 0.08g/LdH2O Co(NO3)2.6H2O 0.05g/LdH2O Adjust PH to 7.1 with 1M NaOH or HCl 说明: 配制母液(1)-(8)号时,药品分别配制,分别存放。(9)号中的药品按量配制后混合存放。 配制工作液时,取(1)号母液10ml,(2)-(8)号母液各1ml,和(9)号母液1 ml,加入蒸馏水中并定容至1000 ml,然后用1 M NaOH 或HCl 调节pH 至7.1,并灭菌20 分钟。 ;. 本文来源:https://www.wddqw.com/doc/c385fd240042a8956bec0975f46527d3250ca6d7.html